| 23:00 | Billericay – Dover Athletic | 2–0 |
| 23:00 | Braintree Town – Tonbridge Angels | 0–4 |
| 23:00 | Dorking Wanderers – Dagenham and Redbridge | 0–1 |
| 23:00 | Farnham Town – AFC Totton | 0–1 |
| 23:00 | Folkestone Invicta – Walton & Hersham | 5–1 |
| 23:00 | Hemel Hempstead – Chelmsford City | 1–1 |
| 23:00 | Maidstone United – Farnborough FC | 1–2 |
| 23:00 | Salisbury City – Hampton & Richmond | 5–0 |
| 23:00 | Torquay – Horsham | 1–1 |
| 23:00 | Truro City – Maidenhead | 2–0 |
| 23:00 | Weston super Mare – Chesham United | 3–0 |
| 23:00 | AFC Telford – Brackley Town | 1–2 |
| 23:00 | Buxton FC – Hereford | 2–1 |
| 23:00 | Chorley FC – Chester | 1–2 |
| 23:00 | Darlington – Worksop Town | 1–2 |
| 23:00 | Hebburn Town – Southport | 3–1 |
| 23:00 | King's Lynn – Harborough Town | 1–1 |
| 23:00 | Marine – Macclesfield | 0–1 |
| 23:00 | Merthyr Town – Hednesford Town | 2–0 |
| 23:00 | Morecambe – Spennymoor Town | 1–1 |
| 23:00 | Oxford City – Bedford Town | 1–0 |
| 23:00 | Radcliffe Borough – Spalding United | 0–2 |
| 23:00 | Scarborough Athletic – South Shields | 0–1 |